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Catalyst One*

Chemistry Analyzer

> OPERATOR’S GUIDE

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Proprietary rights notice

Information in this document is subject to change without notice. Companies, names, and data used in examples

are ctitious unless otherwise noted. No part of this document may be reproduced or transmitted in any form or by

any means, electronic, mechanical, or otherwise, for any purpose, without the express written permission of IDEXX

Laboratories. IDEXX Laboratories may have patents or pending patent applications, trademarks, copyrights, or other

product and company names and logos are trademarks of their respective holders.

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Contents

Preface ...................................................................................................................................................... 5

Safety Precautions ..................................................................................................................................................... 5

Performance Precaution........................................................................................................................................... 5

Care of the Analyzer .................................................................................................................................................. 5

International Symbol Descriptions ......................................................................................................................... 6

Other Symbols ............................................................................................................................................................ 7

Getting Started .......................................................................................................................................... 8

Introduction ................................................................................................................................................................. 8

Using the Catalyst One* Analyzer ............................................................................................................. 14

Analyzing Samples ..................................................................................................................................................14

Slide Handling ........................................................................................................................................................... 14

Diluting Samples ......................................................................................................................................................14

Viewing and Printing Test Results .......................................................................................................................16

Outside of Reportable Range Samples ...............................................................................................................16

Modifying the Settings on the Analyzer ................................................................................................... 18

Modifying the Sound Settings

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..............................................................................................................................18

Entering Standby Mode...........................................................................................................................................18

Sample Storage ........................................................................................................................................................23

Quality Control ......................................................................................................................................... 24

Overview ....................................................................................................................................................................24

Quality Control Materials .......................................................................................................................................24

Quality Control CLIPs and Slides ...........................................................................................................................25

Preparing Control Fluid ...........................................................................................................................................26

Running Quality Control ..........................................................................................................................................27

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Maintenance ............................................................................................................................................ 28

Overview ....................................................................................................................................................................28

Upgrading the Software .......................................................................................................................................... 28

Medical Protocol Descriptions ..............................................................................................................................55

Differences in Results .............................................................................................................................................60

Technical Specications .........................................................................................................................................60

IDEXX Customer and Technical Support contact information........................................................................ 61

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Preface

Note: If the equipment is used in a manner other than specied, the protection provided by the equipment

may be impaired.

The analyzer does not contain any user-serviceable components. DO NOT disassemble.

Line voltage for the Catalyst One AC power adapter is 100–240 V AC, 50–60 Hz. Be sure to plug all

equipment into properly grounded electrical outlets.

+ If you suspect that your analyzer needs service or repair.

+ Whenever you clean the case.

Do not use certain liquids, aerosols (such as canned air), solvents, ammonia, and other substances on or

near the analyzer which could inuence results.

It is recommended that you do not stack other equipment or containers on top of the analyzer.

Keep analyzer away from sources of heat or ames.

PROTECT your equipment from damp conditions, wet weather, or liquid spills.

Take care not to spill water or other liquids on the unit.

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information related to the product (such as expiration date, temperature limitations, batch code, etc.). IDEXX

Laboratories has adopted the use of international symbols on our analyzers, product boxes, labels, inserts,

and manuals in an effort to provide our users with easy-to-read information.

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Getting Started

Welcome to IDEXX’s next-generation chemistry analyzer—the Catalyst One* Chemistry Analyzer.

recheck values over time, customize proles by adding single tests to CLIPs. You can even run up to 25

The Catalyst One analyzer is for veterinary use only.

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patient’s record. The diagnostic results report is a comprehensive report of all the test results specied in a

laboratory request for that patient on a specic day.

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1 µg/mL = 4.31 µmol/L

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Modifying the Settings on the

Analyzer

The analyzer will beep when it encounters an alert. You can modify the Sound settings to turn the sound off

or adjust its volume.

You can modify the settings of the analyzer so that it enters Standby mode at a certain time each day or

put it in Standby mode immediately.

1. Tap the Catalyst One icon on the IDEXX VetLab Station Home screen.

2. If you do not want the analyzer to ever enter Standby mode, tap Never in the Standby area.

3. If you want the analyzer to enter Standby mode at a certain time each day, tap Daily in the Standby area

and then select the desired start time from the available drop-down list.

4. If you want the analyzer to enter Standby mode immediately, tap Now in the Standby area.

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Feature coming soon

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Sample Preparation and Storage

The following sample types can be used with Catalyst* CLIPs and slides:

Serum

Lithium Heparin-

Treated Plasma

Fluoride/

Oxalate-Treated

Whole Blood

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Serum

Treated Plasma

Fluoride/

Oxalate-Treated

Whole Blood

You can run untreated whole blood, lithium heparinized whole blood, plasma, serum, and urine samples on

the Catalyst One analyzer.

To Prepare an Untreated Whole Blood Sample

3. Gently swirl (do not invert or shake) the whole blood separator at least 5 times to mix the sample with

Caution: Ensure that the cap is removed before loading the separator into the analyzer.

To Prepare a Plasma Sample

1. Use the appropriate tube and collection device.

2. Draw the sample gently and transfer if necessary.

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5. Immediately after centrifugation, use a transfer pipette (or a 300 µL pipette) to transfer the appropriate

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combinations. The following table provides general guidance for tests that do not include a reagent

consumable. Refer to applicable quick reference guides for test-specic sample type and volume

requirements.

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+ Mix the samples gently, but thoroughly, by inversion. Do not shake.

+ Centrifuge the samples to remove any brin particles (or urine sediment) that may have formed during

storage.

+ Analyze the samples immediately after centrifugation.

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Quality Control

The purpose of quality control (QC) is to verify the integrity of your slides and also to verify that your

Catalyst One* analyzer is functioning properly.

You should run a QC test:

+ When the analyzer is rst installed.

+ After cleaning the internal components of the analyzer.

+ If the analyzer has been moved.

+ To verify system performance.

VetTrol* Control

Expired or unwanted material should be discarded with other clinical waste.

Note: Do not store in the freezer door; only in the main freezer compartment.

For most chemistries, VetTrol Control can be used up to 24 hours after reconstitution when it is stored in

the refrigerator and equilibrated to room temperature before running (do not leave at room temperature for

more than 2 hours). For creatine kinase and ammonia values, VetTrol Control uid should be used within

2 hours following reconstitution. Exposure to light will affect total bilirubin and creatine kinase results.

Ammonia concentration will increase with time.

UPRO Control

In each box of UPRO Control, there are six vials containing the control uid. The lot number can be found on

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In each box of Advanced Control, there is one vial containing the control uid. The lot number can be found

on the product packaging.

Note: Each vial contains enough uid for 2 runs, in the event a secondary run is necessary.

Store frozen until the expiration date, or store in the refrigerator for up to 5 days.

Once opened, Advanced Control cannot be stored and reused—discard remaining uid after use.

PHBR Control

In each box of PHBR Control, there are six vials containing the control uid. The lot number can be found on

the product packaging.

components of your analyzer. The convenient Catalyst* QC CLIP contains all of the chemistry slides

needed to perform this task. It is also recommended that you perform a quality control for electrolytes using

Run the QC CLIP and the Lyte 4 CLIP

Use the convenient QC CLIP and the Lyte 4 CLIP in conjunction with the VetTrol Control uid to perform

quality control on your Catalyst One analyzer. It is recommended that you wait at least 30 minutes after

running any slides before running the QC CLIP.

You can use individual slides to create your own QC panel and perform a quality control test (one slide per

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Principal Reason for Performing the Test

As an indicator of hepatic and/or biliary disease.

reduce ALKP results.

Complementary Tests

Alkaline phosphatase activity is usually determined in conjunction with other tests of hepatic function and

damage.

)

Ammonia is the catabolic product of protein digestion and is extremely toxic. It is converted rapidly in the

liver to urea, which is eliminated from the body by the kidneys.

Principal Reason for Performing the Test

recommended as the sample of choice.

Ammonia measurements in either plasma or serum are signicantly affected by environmental factors

and/or the passage of time. Minimal exposure of the sample to the air is essential. All sample containers

should be capped unless sample is being introduced or withdrawn. Do not attempt to measure ammonia in

hemolyzed samples. Contamination from the red blood cells will invalidate the test.

Complementary Tests

Ammonia may be determined in isolation but more often in conjunction with other tests of hepatic damage

or dysfunction, such as pre- and postprandial bile acids.

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Most Common Abnormality Indicated by the Test

Acute necrotizing pancreatitis.

Sample Type and Precautions

Remove plasma or serum promptly from the cells or clot. Hemolyzed specimens should not be used. Do

not use oxalate, citrate, or EDTA anticoagulants. If plasma is being collected, use only lithium heparinized

samples.

Blood samples should be taken within one day of the onset of symptoms that suggest acute pancreatitis.

Complementary Tests

Amylase and lipase are usually determined in conjunction with one another. Evaluation of a comprehensive

chemistry prole that includes electrolytes is generally recommended because of secondary effects of

the blood during cell injury. If no increase in ALT is seen in conjunction with an increased AST in the dog

and cat, cardiac or skeletal muscle cell injury is most likely. For increased AST values with equine, bovine,

and porcine samples, liver, cardiac, and skeletal muscle cell injury must be considered.

Principal Reason for Performing the Test

To investigate damage to liver, cardiac, or skeletal muscle.

Most Common Abnormalities Indicated by the Test

Dogs and cats—cardiac or skeletal muscle injury when ALT is not increased; liver, cardiac, or skeletal

muscle injury if both ALT and AST are increased.

Horses, cows, and pigs —liver, cardiac, or skeletal muscle injury.

Sample Type and Precautions

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Blood samples should be processed and centrifuged immediately after collection. Even slight hemolysis

skeletal muscle function or damage.

Bile Acids (BA)

Bile acids are produced in the liver, stored in the gallbladder, and released into the intestinal tract where

they aid in lipid digestion. In healthy animals, bile acids are eciently reabsorbed from the intestinal tract

and recirculated to the liver via the portal vein. Once in the liver, bile acids are removed from circulation by

the hepatocytes. In states of disease or abnormal portal blood ow, bile acids can become elevated in the

systemic circulation, indicating reduced liver function.

Principal Reason for Performing the Test

Bile acids testing is primarily used to evaluate for loss of liver function or presence of portosystemic

Most Common Abnormalities Indicated by the Test

Elevated pre- and/or postprandial bile acids are suggestive of liver dysfunction. Normal bile acids do not

rule out the presence of hepatic disease. Mild elevations may also be seen with extrahepatic diseases (e.g.,

small intestinal bacterial overgrowth [SIBO], hyperadrenocorticism, etc.). Moderate to severe elevations are

consistent with hepatic dysfunction but cannot discriminate specic liver diseases or the relative severity or

reversibility of liver disease. For additional information see the Bile Acids differentials in VetConnect* PLUS.

Sample Types and Precautions

Catalyst Bile Acids supports the use of serum, lithium heparin plasma, and whole blood (using the Catalyst

Lithium Whole Blood Separator). Remove plasma or serum promptly from the cells or clot. IDEXX does not

recommend freezing samples that will be used to run Catalyst Bile Acids.

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+ Catalyst Bile Acids is robust to lipemia.

stimulation protocols is recommended to increase sensitivity. The following bile acids stimulation protocol

is recommended:

1. Fast the dog or cat for approximately 12 hours and collect a fasting (preprandial) sample. Obtain a result

from the preprandial Catalyst Bile Acids test.

2. Feed the animal a small amount of high-fat food to stimulate gallbladder contraction.

– The minimum amount of food recommended is 2 tsp for small patients (<10 lb) and

2 tbsp for large patients.

– If encephalopathic effects of protein are anticipated, use a restricted-protein food mixed with a small

amount of corn oil.

3. Two hours after feeding, collect a postprandial sample. Obtain a result from the postprandial Catalyst

+ Chemistry (decreased albumin, BUN, glucose, or cholesterol; increased ALT, AST, ALKP, GGT, or

+ Urinalysis (ammonium biurate crystalluria)

If bilirubin concentration is elevated or the animal is icteric, there is little additional value in performing a bile

acids test.

Blood Urea Nitrogen (BUN)

The catabolism of proteins results in the production of ammonia, which is extremely toxic. Ammonia is

converted to urea in the liver and eliminated from the body by glomerular ltration in the kidneys.

Principal Reason for Performing the Test

As an indicator of renal disease or pathologic conditions that result in bleeding into the gastrointestinal

tract.

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Most Common Abnormalities Indicated by the Test

Increased urea—prerenal, postrenal, and renal azotemia with decreased glomerular ltration rate; high-

protein diet or bleeding into the gastrointestinal tract.

Decreased urea —decreased protein intake; hepatic insuciency; diuresis.

Sample Type and Precautions

inorganic phosphate, total protein, albumin, and a complete urinalysis. Urea concentration is inuenced by

high-protein diet rather than creatinine.

Calcium (Ca)

Calcium is an essential element that is involved in many body systems. These include the skeleton, enzyme

activation, muscle metabolism, blood coagulation, and osmoregulation. In the blood, calcium exists in

ionized and protein bound forms. Factors governing the total plasma, whole blood, or serum concentration

are complex and include interaction with other chemical moieties, proteins, and hormones.

Principal Reason for Performing the Test

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Complementary Tests

Calcium should be determined in conjunction with measurements of inorganic phosphate, albumin, total

protein, and glucose. Ionized calcium measurement will provide more specic information related to the

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Most Common Abnormality Indicated by the Test

Increased cholesterol—hypothyroidism, postprandial, nephrotic syndrome.